Newcastle Disease Vaccine, B1 Type, LaSota Strain, Live Virus
Newcastle disease is an avian pathogen causing severe economic losses to the Indian poultry industry due to recurring outbreaks in vaccinated and unvaccinated flocks. India being an endemic country, advocates vaccination against the virus using lentogenic and mesogenic strains. Two virus strains which are commonly used for vaccination are strain F a lentogenic virus and strain R2B a mesogenic virus.
Strain F is given to 0—7 days old chicks and R2B is given to older birds which are around 6—8 weeks old. To understand the genetic makeup of these two strains, a complete genome study and phylogenetic analysis of the F, HN genes of these vaccine strains were carried out.
Both the viral strains had a genome length of 15, nucleotides and consisted of six genes with conserved complimentary 3' leader and 5' trailer regions. Although both the viral strains had different virulence attributes, the length of the HN protein was similar with amino acids.
Phylogenetic analysis of F, HN and complete genome sequences grouped these two strains in genotype II category which are considered as early genotypes and corroborated with their years of isolation. Newcastle disease virus NDVthe prototype of paramyxovirus, causes the highly contagious Newcastle disease ND in many avian species, resulting in substantial economic losses in the poultry industry worldwide. Strains of NDV are classified into three main pathogens as highly virulent velogenicintermediate mesogenic and non-virulent lentogenic on the basis of their pathogenicity for chickens.
NDV is a member of the Avulavirus genus in the Paramyxovirus family . NDV genome is approximately 15 kb long, non-segmented, single-stranded, negative-sense RNA that codes for six proteins: nucleoprotein NPphosphoprotein Pmatrix M protein, fusion F protein, haemagglutinin-neuraminidase HN protein, and polymerase L protein .
Two different systems classifying NDV are currently used worldwide. Vaccination of commercially reared birds is the only way to reduce disease and the losses resulting from infection.
India, being an endemic country for NDV, outbreaks still occurs in spite of regular vaccination programmes. Though many reasons could be attributed to this scenario, presence of the etiological agent in the vicinity may always pose a severe threat even to vaccinated population. This gains importance by the fact that many of the free-roaming local birds, water fowls and wild birds are reported to harbour velogenic NDV without manifesting clinical signs .
Since then, in several countries in Europe, Africa and Asia, the use of the virus as an immunizing agent in the form of a live vaccine has been studied . The virus strain had originated by passaging three Indian field isolates in embryonated chicken eggs, with one of the lines showing signs of attenuation after 19 passages . This virus had undergone 8 serial passages in embryonated eggs in April when it was received at this Institute.The disease in animals is characterized by abortion, retained placenta and to a lesser extent, orchitis and infection of the accessory sex glands in males.
Brucellosis primarily affects cattle, buffalo, bison, pigs, sheep, goats, dogs, elk and occasionally horses. The disease in man is called Undulant Fever. Control and elimination of this disease in cattle involves testing and culling reactors and vaccinating healthy females.
It has been used exclusively since than as part of the federal program to eradicate brucellosis from the United States because it is safe, effective and does not interfere with diagnostic testing for infection. Brucella Abortus Vaccine, RB, is the result of research, development and production efforts by both the public and private sectors.
It was selected as a stable strain which produces protective immunity while not inducing antibodies which react in standard serological tests. RB has been tested to verify safety, efficacy and to demonstrate that there is no serological reaction in standard tests. The Animal and Plant Health Inspection Service of the US Department of Agriculture with cooperation of the Centers for Disease Control conducted a risk analysis to assess the potential effect of this product on the safety of animals, public health, and the environment.
Distribution in the United States shall be limited to authorized recipients designated by proper state officials under such additional conditions as these authorities may require. For use in healthy female cattle 4 to 12 months of age as an aid in the prevention of infection and abortion caused by Brucella abortus. Do not vaccinate within 3 weeks before slaughter. Rehydrate with accompanying vial of sterile diluent supplied as a component part of this package.
Shake well after rehydration. Administer 2.R2B ভ্যাকসিন কেনেকৈ দিব লাগে। Barpeta Assam 9365429701 / 7002442862
Anaphylactoid reaction may occur following administration of products of this nature. If noted, administer adrenalin or equivalent. In the case of accidental human exposure contact a physician. Warning: This organism is Rifampin and Penicillin resistant. Diluent is buffered solution specifically prepared for use with this vaccine. Use only this diluent to assure viability of the vaccine. The easiest way to lookup drug information, identify pills, check interactions and set up your own personal medication records.
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Subscribe to Drugs. This material is provided for educational purposes only and is not intended for medical advice, diagnosis or treatment. We comply with the HONcode standard for trustworthy health information - verify here. Skip to Content. For use by or under the supervision of a veterinarian. Directions For use in healthy female cattle 4 to 12 months of age as an aid in the prevention of infection and abortion caused by Brucella abortus.
Do not administer to pregnant cows. Precautions Anaphylactoid reaction may occur following administration of products of this nature. Use entire contents when first rehydrated. Burn, autoclave or chemically disinfect this container and all unused contents. Conveniently packaged in 25 dose, and 5 dose sizes. USDA Code License No. Phone: www. Fine Veterinary Biologics since Every effort has been made to ensure the accuracy of the Brucella Abortus Vaccine Strain RB information published above.
However, it remains the responsibility of the readers to familiarize themselves with the product information contained on the US product label or package insert.Newcastle disease virus NDVstrain R2B is a mesogenic vaccine strain used for booster vaccination in chickens against Newcastle disease in India and many south East Asian countries.
A full-length cDNA clone of the virus was generated by ligating eight overlapping fragments generated by reverse transcription polymerase chain reaction having unique restriction enzyme sites within them.
This full-length cDNA clone was flanked by hammerhead ribozyme and hepatitis delta virus ribozyme sequences.
Defined genetic markers were introduced into the NDV genome to differentiate the rescued virus from the parent virus. A gene cassette containing the reporter gene, green fluorescent protein flanked by NDV gene-start and gene-end signals was generated by PCR and introduced into the full-length clone of NDV between the P and M genes. Recombinant NDV encoding the GFP gene was rescued having precise termini when transfected into permissive Vero cells along with support plasmids harbouring the nucleoprotein, phosphoprotein and polymerase genes.
The recombinant virus had similar growth kinetics as that of the parent virus with a moderate reduction in the virulence. The generation of reverse genetics system for NDV strain R2B will help in the development of multivalent vaccines against viral diseases of livestock and poultry.
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Lomniczi, Virus Res. Lamb, D. Kolakofsky, In: D. Knipe, P. Howley eds. Fields Virology, 4th ed, pp. Lippincott Williams and Wilkins, Philadelphia Desingu, S. Singh, K.
poultry LIVE VACCINES
Dhama, O. Vinodhkumar, R. Barathidasan, Y. Mailk, R. Singh, R.Virotherapy is emerging as an alternative treatment of cancer. Tumor growth inhibition was compared with their respective control groups by gross volume and histopathological evaluation.
Histopathological examination revealed that the virus caused apoptosis, followed by necrosis, but immune cell infiltration was not remarkable.
Rescue of a recombinant Newcastle disease virus strain R2B expressing green fluorescent protein
In conclusion, the test NDV strain was found to be safe and showed oncolytic activity against the SW cell line in mice. This is a preview of subscription content, log in to check access.
Rent this article via DeepDyve. Viruses — Int J Poultry Sc — Mol Ther — Open Immunol J — J Virol — Oncol Virotherap — PLoS One 9:e J Transl Med Gene Ther — Virus Res — PLoS One.
J Oncol. Hastie E, Grdzelishvili VZ Vesicular stomatitis virus as a flexible platform for oncolytic virotherapy against cancer. J Gen Virol — Clin Cancer Res — Mole Ther — J Virol Methods — Appl Biochem Biotechnol — Kumar U, Kumar S Molecular characterization of an apoptotic strain of Newcastle disease virus isolated from an outbreak in India.
Can Gene Ther — J Biomed Biotechnol. Head Neck — Cancer Res — J Natl Cancer Inst — Mole Ther Oncolytics Cancer Gene Ther — J Trans Med.During these challenging times, we guarantee we will work tirelessly to support you. We will continue to give you accurate and timely information throughout the crisis, and we will deliver on our mission — to help everyone in the world learn how to do anything — no matter what.
Thank you to our community and to all of our readers who are working to aid others in this time of crisis, and to all of those who are making personal sacrifices for the good of their communities. We will get through this together. Updated: April 12, Reader-Approved References. If you own chickens — be it 3 or 3, — you will need to vaccinate them in order to keep them healthy.
Together, they cited information from 23 references. It also received 13 testimonials from readers, earning it our reader-approved status. Learn more Explore this Article Getting Started with Vaccines. Vaccinating with a Subcutaneous Injection. Administering Eye Drop Vaccines. Mixing Vaccines in Drinking Water. Preparing a Wing Web Vaccination. Cleaning Up After Vaccinations. Show 3 more Show less Things You'll Need.
Tips and Warnings. Related Articles.Chickens are susceptible to many infectious diseases. One of the most important of these is the viral disease known as Newcastle disease, which causes devastating losses in both commercial and village chickens. Reducing losses of large numbers of village chickens to virulent Newcastle disease is an essential first step to improving their productivity.
Newcastle disease can be controlled by the use of vaccines. There are many Newcastle disease vaccines suitable for use in commercial chickens. These are available on the international market. The I-2 Newcastle disease vaccine has been developed for local or regional production and use in controlling Newcastle disease in village chickens.
Many Newcastle disease vaccines deteriorate after storage for one or two hours at room temperature. This makes them unsuitable for use in villages where the vaccine may need to be transported for hours or in some cases days at ambient temperature. The I-2 Newcastle disease vaccine is more robust and is known as a thermostable vaccine.
Brucella Abortus Vaccine (Strain RB-51)
Thermostable vaccines still require long-term storage in the refrigerator. However during transportation of the vaccine to the field, the vaccine will not deteriorate as quickly as the traditional vaccines. Evaporative cooling provided by wrapping the vaccine in a damp cloth will be adequate for maintaining the viability of the vaccine during transportation to remote villages.
Chickens that survive infection with virulent Newcastle disease virus develop a long lasting immunity to further infection with Newcastle disease virus. Circulating antibodies. Secreted antibody producing mucosal immunity. Cell mediated immunity. Newcastle disease virus of low virulence induces similar immune responses without causing severe disease.
This is the basis of vaccination. These vaccines are made with virus that is alive and able to infect cells. Strains of virus of low or moderate virulence are used. They mimic natural infection and induce all three immune responses.
The ability of the virus to infect cells has been destroyed by treatment with a chemical, radiation or heat. These vaccines invoke only a circulating antibody response. Strains of Newcastle disease virus have been broadly classified into four pathotypes as follows:. Many strains of Newcastle disease virus other than velogenic strains are used in the production of live vaccines.
Eight of these strains are listed in Table 1. Usually used in young chickens but suitable for use as a vaccine in chickens of all ages. Often causes post vaccination respiratory signs, used as a booster vaccine in flocks vaccinated with F or B1. An invasive strain, used as a booster vaccine. Can cause adverse reactions respiratory distress, loss of weight or drop in egg production and even death if used in partially immune chickens. Usually administered by injection.
Less pathogenic than Mukteswar, used as booster vaccine. Thermostable Newcastle disease vaccines exhibit a relative resistance to inactivation on exposure to elevated temperatures. Strains of Newcastle disease virus vary in thermostability. Thermostable vaccines are prepared from a strain of Newcastle disease virus that retains its ability to infect cells after storage outside a cold chain for a short period of time.
There are two basic processes used to produce a thermostable Newcastle disease vaccine. A seed lot system is used to produce the vaccine. The sequential use of a master seed and a working seed minimizes the number of passages to produce a vaccine and maintains the genetic stability of the vaccine virus.The originating document has been archived. We cannot confirm the completeness, accuracy and currency of the content. These Newcastle Disease Vaccines accompanied by diluent are recommended for the vaccination of healthy chickens one day of age or older.
Remove seal and stopper from vaccine and diluent vials. Avoid contamination of stoppers and contents. Add diluent to half-fill the vaccine vial. Replace stopper and shake until contents are dissolved. Do not release bird until vaccine has been inhaled.
These Newcastle Disease Vaccines are recommended for the vaccination of healthy chickens 2 weeks of age or older. Discontinue use of medications or sanitizing agents in the drinking water 24 hours before vaccinating. Do not resume use for 24 hours following vaccination. Water used for the drinking-water administration of a live virus vaccine must be non-chlorinated. Provide enough waterers so two-thirds of the birds may drink at one time. Scrub waterers, with fresh, clean, non-chlorinated water, and use no disinfectant.
Let the waterers drain dry. Turn off automatic waterers, so the only available water is the vaccine water. Do not give vaccine water through medication tanks. Withhold water for 2 hours before vaccinating.
Do not deprive the birds of water if the temperature is extremely high. Distribute the final volume of vaccine water evenly among the clean waterers. Do not place the waterers in direct sunlight. Resume regular water administration only after all the vaccine water has been consumed. These Newcastle Disease Vaccines may be used for the revaccination of healthy chickens 4 weeks of age or older by spraying the vaccine solution above the chickens.
A sprayer that delivers a coarse spray quickly and evenly is recommended. Pour rehydrated vaccine into a clean container and add cool, distilled water per chart below and shake thoroughly. Direct the spray above the heads of the birds.
See chart below:. This spray method of vaccination should be employed in poultry houses where air movement can be reduced to a minimum.